Journal: bioRxiv

Article Title: A combinatorial EVs-miRNA signature mediates the anti-tumoral activity of NFAT3-regulated extracellular vesicles in aggressive cancers

doi: 10.64898/2026.02.13.702809

Figure Lengend Snippet: (A) Hetero-spheroids growth and invasion assays performed in TNBC cell lines (MDA-MB 231, SUM-159-PT) and the pancreatic cancer cell line (MIA PaCa-2) following transfection with the 15-miRNA combination (miR-Comb 15) or a control miRNA (miR-Ctl). (B) Comparative analysis of hetero-spheroids growth and invasion following transfection with miR-Comb 15 or a reduced 11-miRNA combination (miR-Comb 11). Each dot represents an independent experiment; bars indicate the mean. (C) Left panel: representative immunofluorescence images of MDA-MB-231 and MIA PaCa-2 cells transfected with miR-Ctl or miR-Comb 15, stained for F-actin (phalloidin, magenta) and nuclei (DAPI, blue), showing changes in cell morphology and cell area, size bar scale represents 20μm. Right panel: Quantification of cell area from immunofluorescence images. Each dot represents a single cell. Each dot in in A and B represents an independent experiment; bars indicate the mean. Statistical significance was determined by an unpaired two-tailed Student’s t-test using GraphPad Prism: ns, not significant; *p<0.05, **p<0.005, ***p<0.0005, ****p<0.0001 versus miR-Ctl. Data in (C) represent pooled single-cell measurements. Statistical significance was determined using an unpaired two-tailed Student’s t-test: ****p < 0.0001versus miR-Ctl.

Article Snippet: After blocking with PBS-Tween containing 3% BSA, cells were incubated with Alexa Fluor 488 phalloidin (Cell Signaling Technology, #8878) for 1 h, followed by DAPI nuclear staining (Cell Signaling Technology, #62248).

Techniques: Transfection, Control, Immunofluorescence, Staining, Single Cell, Two Tailed Test